Simultaneous Adsorption of Intact Glycoproteins and Phosphoproteins Using Hydrophilic Titanium (IV) Ion-Immobilized Cotton Fiber Functionalized by Phytic Acid Assembly

Protein glycosylation and phosphorylation are two important post-translational modifications play significant roles in various biological processes. However, both glycoproteins phosphoproteins low abundance complex samples, which hinders their characterization. In this work, we developed a novel method for the simultaneous adsorption of based on solid phase extraction. By virtue unique structure hydrophilic titanium (IV) ion immobilized cotton fiber functionalized with phytic acid (denoted as Ti4+-PA-cotton), glycoproteins, could be efficiently enriched one-step incubation eluted sequence. The newly prepared Ti4+-PA-cotton was well characterized, isotherms kinetics were studied. maximum capacities β-casein HRP 833.3 mg/g 384.6 rate 259.1 63.1 mg/g·min. Standard protein mixture BSA, HRP, used to test enrichment ability proof concept. SDS-PAGE results demonstrated that had an excellent performance possessed outstanding application potential analysis phosphoproteins.